Distribution of tet(H) among Pasteurella isolates from the United States and Canada

Author:

Hansen L M1,Blanchard P C1,Hirsh D C1

Affiliation:

1. Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis 95616, USA.

Abstract

Tetracycline-resistant isolates of Pasteurella multocida and Pasteurella haemolytica obtained from various locations in the United States and Canada were studied to determine the distribution of the tet(H) gene. Of the 31 isolates examined, 25 were found to contain the tet(H) gene. Chromosomal or plasmid DNA obtained from those that did not contain the tet(H) gene did not hybridize with probes specific for classes A through G, though chromosomal DNA from one isolate lacking tet(H) hybridized with a probe specific for class M. The tet(H) gene was found on plasmid as well as on chromosomal DNA, suggesting that it is carried on a transposable element.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Reference39 articles.

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3. Ausubel F. M. R. Brent R. E. Kingston D. D. Moore J. G. Seidman J. A. Smith and K. Struhl. 1987. Current protocols in molecular biology. John Wiley & Sons New York.

4. Complete sequence of pSC101;Bernardi A.;Nucleic Acids Res.,1984

5. Heterogeneity of tetracycline resistance determinants in Streptococcus;Burdett V.;J. Bacteriol.,1982

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