Expressionof Unmodified Hepatitis C Virus Envelope Glycoprotein-Coding SequencesLeads to Cryptic Intron Excision and Cell Surface Expression of E1/E2Heterodimers Comprising Full-Length and Partially DeletedE1-Reference-Cited by-同舟云学术

Expressionof Unmodified Hepatitis C Virus Envelope Glycoprotein-Coding SequencesLeads to Cryptic Intron Excision and Cell Surface Expression of E1/E2Heterodimers Comprising Full-Length and Partially DeletedE1

Published:2003-12-15 Issue:24 Volume:77 Page:13418-13424
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Dumonceaux Julie¹,Cormier Emmanuel G.¹²,Kajumo Francis¹²,Donovan Gerald P.²,Roy-Chowdhury Jayanta¹,Fox Ira J.³,Gardner Jason P.²,Dragic Tatjana¹

Affiliation:

1. Albert Einstein College of Medicine, Bronx, New York 10461

2. Progenics Pharmaceuticals, Inc., Tarrytown, New York 10591

3. Department of Surgery, Nebraska Medical Center, Omaha, Nebraska 98198

Abstract

ABSTRACT Hepatitis C virus (HCV) is a positive-strand RNA virus that replicates exclusively in the cytoplasm of infected cells. The viral envelope glycoproteins, E1 and E2, appear to be retained in the endoplasmic reticulum, where viral budding is thought to occur. Surprisingly, we found that the expression system used to generate HCV envelope glycoproteins influences their subcellular localization and processing. These findings have important implications for optimizing novel HCV fusion and entry assays as well as for budding and virus particle formation.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.77.24.13418-13424.2003

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