Enhancement of Targeted Homologous Recombination in Mycoplasma mycoides subsp. capri by Inclusion of Heterologous recA-Reference-Cited by-同舟云学术

Enhancement of Targeted Homologous Recombination in Mycoplasma mycoides subsp. capri by Inclusion of Heterologous recA

Published:2010-10-15 Issue:20 Volume:76 Page:6951-6954
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Allam Ayman B.¹,Reyes Leticia¹,Assad-Garcia Nacyra²,Glass John I.²,Brown Mary B.¹

Affiliation:

1. Department of Infectious Diseases and Pathology, College of Veterinary Medicine, University of Florida, Gainesville, Florida

2. J. Craig Venter Institute, Rockville, Maryland

Abstract

ABSTRACT A suicide plasmid, pExp1- ctpA :: tetM - recA ec, employing recA from Escherichia coli and tetM as a selection marker, was used to generate ctpA knockout mutants in Mycoplasma mycoides subsp. capri through targeted gene disruption. Inclusion of E. coli recA greatly enhanced both the consistency and the recovery of mutants generated by homologous recombination.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00056-10

Reference35 articles.

1. Burgos, R., O. Q. Pich, E. Querol, and J. Pinol. 2008. Deletion of the Mycoplasma genitalium MG_217 gene modifies cell gliding behaviour by altering terminal organelle curvature. Mol. Microbiol.69:1029-1040.

2. Phase-locked mutants of Mycoplasma agalactiae: defining the molecular switch of high-frequency Vpma antigenic variation

3. Chopra-Dewasthaly, R., M. Marenda, R. Rosengarten, W. Jechlinger, and C. Citti. 2005. Construction of the first shuttle vectors for gene cloning and homologous recombination in Mycoplasma agalactiae. FEMS Microbiol. Lett.253:89-94.

4. First steps towards the genetic manipulation of Mycoplasma agalactiae and Mycoplasma bovis using the transposon Tn4001mod

5. Identification of the Origin of Replication of the Mycoplasma pulmonis Chromosome and Its Use in oriC Replicative Plasmids

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