Specific Identification of Listeria welshimeri and Listeria monocytogenes by PCR Assays Targeting a Gene Encoding a Fibronectin-Binding Protein

Author:

Gilot Philippe1,Content Jean1

Affiliation:

1. Department of Virology, Pasteur Institute, B-1180 Brussels, Belgium

Abstract

ABSTRACT We have cloned and sequenced a Listeria welshimeri DNA fragment homologous to the previously described fibronectin-binding protein-encoding gene ( fbp ) of Listeria monocytogenes (P. Gilot, Y. Jossin, and J. Content, J. Med. Microbiol., 49:887-896, 2000). This L. welshimeri DNA fragment expresses a 24.8-kDa protein that binds to human fibronectin. Based on the fbp sequences, we developed novel PCR assays for the identification of L. welshimeri and L. monocytogenes .

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference30 articles.

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