Identification of a Novel Mycobacterial 3-Hydroxyacyl-Thioester Dehydratase, HtdZ (Rv0130), by Functional Complementation in Yeast-Reference-Cited by-同舟云学术

Identification of a Novel Mycobacterial 3-Hydroxyacyl-Thioester Dehydratase, HtdZ (Rv0130), by Functional Complementation in Yeast

Published:2008-06 Issue:11 Volume:190 Page:4088-4090
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Gurvitz Aner¹²,Hiltunen J. Kalervo²,Kastaniotis Alexander J.²

Affiliation:

1. Section of Physiology of Lipid Metabolism, Institute of Physiology, Center for Physiology and Pathophysiology, Medical University of Vienna, Vienna, Austria

2. Biocenter Oulu, Department of Biochemistry, University of Oulu, Oulu, Finland

Abstract

ABSTRACT We report on the identification of Mycobacterium tuberculosis HtdZ (Rv0130), representing a novel 3-hydroxyacyl-thioester dehydratase. HtdZ was picked up by the functional complementation of Saccharomyces cerevisiae htd2 Δ cells lacking the dehydratase of mitochondrial type II fatty acid synthase. Mutant cells expressing HtdZ contained dehydratase activity, recovered their respiratory ability, and partially restored de novo lipoic acid synthesis.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.00016-08

Reference16 articles.

1. Autio, K. J., J. L. Guler, A. J. Kastaniotis, P. T. Englund, and J. K. Hiltunen. 2008. The 3-hydroxyacyl-ACP dehydratase of mitochondrial fatty acid synthesis in Trypanosoma brucei. FEBS Lett. 582 : 729-733.

2. Autio, K. J., A. J. Kastaniotis, H. Pospiech, I. J. Miinalainen, M. S. Schonauer, C. L. Dieckmann, and J. K. Hiltunen. 2007. An ancient genetic link between vertebrate mitochondrial fatty acid synthesis and RNA processing. FASEB J. 22 : 569-578.

3. Mutations releasing mitochondrial biogenesis from glucose repression in Saccharomyces cerevisiae

4. Brody, S., C. Oh, U. Hoja, and E. Schweizer. 1997. Mitochondrial acyl carrier protein is involved in lipoic acid synthesis in Saccharomyces cerevisiae. FEBS Lett. 408 : 217-220.

5. Brown, A. K., A. Bhatt, A. Singh, E. Saparia, A. F. Evans, and G. S. Besra. 2007. Identification of the dehydratase component of the mycobacterial mycolic acid-synthesizing fatty acid synthase-II complex. Microbiology 153 : 4166-4173.

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