In vitro synthesis of infectious retroviral RNA-Reference-Cited by-同舟云学术

In vitro synthesis of infectious retroviral RNA

Published:1988-05 Issue:5 Volume:62 Page:1827-1831
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Flamant F¹,Sorge J A¹

Affiliation:

1. Department of Basic and Clinical Research, Research Institute of Scripps Clinic, La Jolla, California 92037.

Abstract

A plasmid was constructed in which a T7 RNA polymerase promoter was placed upstream of a recombinant amphotropic retrovirus genome containing a selectable neomycin resistance gene. To test the infectivity of the RNA produced by T7 RNA polymerase in vitro, the RNA was microinjected into the nuclei of psi 2 packaging cells. Infectious particles conferring G418 resistance were released.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jvi.62.5.1827-1831.1988

Reference13 articles.

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3. Activation of cellular onc gene by promoter insertion in ALV induced lymphoid leukosis;Hayward H. S.;Nature (London),1981

4. Precise localization of m6A in Rous sarcoma virus RNA reveals clustering of methylation sites: implication for RNA processing;Kane S. E.;Mol. Cell. Biol.,1985

5. Expression of a foreign gene in myeloid and Iymphoid progency of common hemopoietic precursors;Keller G.;Nature (London),1985

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