Affiliation:
1. Department of Genetics, The University of Sheffield, Sheffield S10 2TN, England
Abstract
Mutants of the genes
nys1
and
nys3
differ from sensitive strains (
nys
+
) in their sterol content. Ultraviolet absorption spectra of the nonsaponifiable material extracted from cells of
nys
+
demonstrated the presence of ergosterol and 24(28)-dehydroergosterol. In
nys1
mutants, the spectrum suggests the presence of a new sterol. The absorption spectrum of extracts from
nys3
mutants indicates absence of both ergosterol and 24(28)-dehydroergosterol and presence of another new sterol. Conversion of
nys
+
and
nys3
to petite results in loss of 24(28)-dehydroergosterol in the former and the new sterol in the latter, whereas the new sterol in
nys1
is only reduced. The sterols in ethanol-grown cells of all genotypes are essentially the same as is found for growth on glucose. With the exception of
nys3
grown on ethanol, the mutants do not appear to be at a disadvantage compared to wild type.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
139 articles.
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