In Vivo and In Vitro Action of New Antibiotics Interfering with the Utilization of N -Acetyl-Glucosamine- N -Acetyl-Muramyl-Pentapeptide

Author:

Lugtenberg E. J. J.1,Van Schijndel-Van Dam Arna1,Van Bellegem T. H. M.1

Affiliation:

1. Laboratory for Microbiology, State University, Catharijnesingel 59, Utrecht, The Netherlands

Abstract

Recent literature on the antibiotics enduracidin, moenomycin, prasinomycin, and 11.837 RP suggested an interaction with murein synthesis. Incubation of sensitive strains from Bacillus cereus and Staphylococcus aureus in a “wall medium” containing labeled l -alanine showed that all four antibiotics inhibited the incorporation of alanine into murein and gave rise to accumulation of radioactive uridine diphosphate- N -acetyl-muramyl (UDP-MurNAc)-pentapeptide. Peptidoglycan was synthesized when the particulate enzyme of B. stearothermophilus was incubated with the murein precursors UDP- N -acetyl-glucosamine (UDP-GlcNAc) and UDP-MurNAc-pentapeptide. The newly formed polymer was less accessible for lysozyme and more strongly bound to the acceptor than the same product from the Escherichia coli particulate enzyme. After incubation in the presence of penicillin, a greater part of the peptidoglycan was lysozyme sensitive and more loosely bound to the acceptor. The antibiotics enduracidin, moenomycin, prasinomycin, and 11.837 RP inhibited peptidoglycan synthesis by the B. stearothermophilus particulate enzyme. The rate of synthesis of GlcNAc-MurNAc(-pentapeptide)-P-P-phospholipid was independent from the addition of these antibiotics, but its utilization was strongly inhibited. With the present results, it is not possible to distinguish the mechanisms of action of enduracidin, moenomycin, prasinomycin, and 11.837 RP from the mechanisms of action of vancomycin and ristocetin.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference35 articles.

1. Biosynthesis of the peptidoglycan of bacterial cell walls. II. Phospholipid carriers in the reaction sequence;Anderson J.;J. Biol. Chem.,1967

2. Enzymatic synthesis of cell wall mucopeptide in a particulate preparation of Escherichia coli;Araki Y.;Biochem. Biophys. Res. Commun.,1966

3. Archibald A. R. J. Baddiley and N. L. Blumsom. 1968. The teichoic acids. 30:223-253.

4. Best G. K. and N. N. Durham. 1964. Effect of vanco

5. Adsorption of the ristocetins to Bacillus subtilis cell walls;Best G. K.;Antimicrob. Ag. Chemother.,1966

Cited by 59 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3