Affiliation:
1. Institute for Enzyme Research, University of Wisconsin, and Veterans Administration Hospital, Madison, Wisconsin 53705
Abstract
The effect of isoniazid on the protoplasmic viscosity in the H37Ra strain of
Mycobacterium tuberculosis
was determined by using electron spin resonance spectroscopy and a small spin label tempone (2,2,6,6-tetramethylpiperidone-
N
-oxyl radical). Isoniazid (0.5 μg/ml) caused the internal cellular viscosity to increase gradually over the first 15 h of exposure from a rotational correlation time value (
T
c
) of 2.4 × 10
−10
to 3.4 × 10
10
s and then decrease linearly to the control level after 27 h. These results could be interpreted to mean that isoniazid allows a continued and normal synthesis of the protoplasmic components while the rate of increase in the cell volume is reduced. A degradative process may begin after the initial 15-h exposure time, which would cause the reduction in the internal viscosity.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
Reference10 articles.
1. Mode of action of isoniazid;Barclay W. R.;Amer. Rev. Tuberc.,1953
2. A summary and evaluation of spin labels used as probes for biological membrane structure;Keith A. D.;Biochim. Biophys. Acta,1973
3. Viscosity of cellular protoplasm;Keith A. D.;Science,1974
4. Theory of ESR line widths of free radicals;Kivelson D.;J. Chem. Phys.,1960
5. Sterilization of tubercle bacilli by isonicotic acid hydrazide and the incidence of variants resistant to the drug in vitro;Middlebrook G.;Amer. Rev. Tuberc.,1952
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