Utility of the Rapid Antigen Detection Test E. histolytica Quik Chek for the Diagnosis of Entamoeba histolytica Infection in Nonendemic Situations-Reference-Cited by-同舟云学术

Utility of the Rapid Antigen Detection Test E. histolytica Quik Chek for the Diagnosis of Entamoeba histolytica Infection in Nonendemic Situations

Published:2020-10-21 Issue:11 Volume:58 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Yanagawa Yasuaki¹²,Shimogawara Rieko²,Endo Tomoyuki³,Fukushima Rika⁴,Gatanaga Hiroyuki¹⁵,Hayasaka Kasumi⁶,Kikuchi Yoshimi¹,Kobayashi Taiichiro⁷,Koga Michiko⁸,Koibuchi Tomohiko⁸,Miyagawa Toshikazu⁹,Nagata Ayaka¹⁰,Nakata Hirotomo⁹,Oka Shinichi¹⁵,Otsuka Risa¹⁰,Sakai Kazumi¹¹,Shibuya Mami¹⁰,Shingyochi Hiroyuki¹²,Tsuchihashi Etsuko¹⁰,Watanabe Koji¹^ORCID,Yagita Kenji²

Affiliation:

1. AIDS Clinical Center, National Center for Global Health and Medicine, Tokyo, Japan

2. Department of Parasitology, National Institutes of Infectious Diseases, Tokyo, Japan

3. Department of Hematology, Hokkaido University Hospital, Hokkaido, Japan

4. Division of Laboratory Diagnosis, Kumamoto University Hospital, Kumamoto, Japan

5. Joint Research Center for Human Retrovirus Infection, Kumamoto University, Kumamoto, Japan

6. Division of Laboratory and Transfusion Medicine, Hokkaido University Hospital, Hokkaido, Japan

7. Department of Infectious Diseases, Tokyo Metropolitan Cancer and Infectious Diseases Center Komagome Hospital, Tokyo, Japan

8. Research Hospital Division of Infectious Diseases and Applied Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan

9. Department of Hematology, Rheumatology, and Infectious Diseases, Kumamoto University, Kumamoto, Japan

10. Laboratory Testing Department, National Center for Global Health and Medicine, Tokyo, Japan

11. Department of Clinical Laboratory, Tokyo Metropolitan Cancer and Infectious Diseases Center Komagome Hospital, Tokyo, Japan

12. Research Hospital Division of Laboratory Diagnosis, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan

Abstract

Entamoeba histolytica infection is an increasingly common sexually transmitted infection in Japan. Currently, stool ova and parasite examination (O&P) is the only approved diagnostic method. Here, we assessed the utility of the commercially available rapid antigen detection test (Quik Chek) for E. histolytica . A multicenter cross-sectional study was conducted. Stool samples that had been submitted for O&P were included. The samples were subjected to both Quik Chek and PCR, and the Quik Chek results were assessed in comparison with PCR as the reference standard.

Funder

Health Labor Sciences Research Grant

Japan Agency for Medical Research and Development

National Center for Global Health and Medicine

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01991-20

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