Inhibition of Antiassociation Activity of Translation Initiation Factor 3 by Paromomycin-Reference-Cited by-同舟云学术

Inhibition of Antiassociation Activity of Translation Initiation Factor 3 by Paromomycin

Published:2007-01 Issue:1 Volume:51 Page:175-180
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Hirokawa Go¹²,Kaji Hideko¹,Kaji Akira²

Affiliation:

1. Department of Biochemistry and Molecular Biology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania 19107

2. Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104

Abstract

ABSTRACT The effect of paromomycin on the interaction of ribosomal subunits was studied. Paromomycin inhibited the antiassociation activity of initiation factor 3 (IF3). Furthermore, ribosomal subunits were associated to form 70S ribosomes by paromomycin even in the presence of 1 mM Mg 2+ . Paromomycin did not inhibit the binding of IF3 to the 30S ribosomal subunits. On the other hand, IF3 bound to the 30S subunits was expelled by paromomycin-induced subunit association (70S formation). These results indicate that the stabilization of 70S ribosomes by paromomycin may in part be responsible for its inhibitory effects on translocation and ribosome recycling.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.01096-06

Reference41 articles.

1. Amarantos, I., I. K. Zarkadis, and D. L. Kalpaxis. 2002. The identification of spermine binding sites in 16S rRNA allows interpretation of the spermine effect on ribosomal 30S subunit functions. Nucleic Acids Res.30:2832-2843.

2. Cabanas, M. J., D. Vazquez, and J. Modolell. 1978. Inhibition of ribosomal translocation by aminoglycoside antibiotics. Biochem. Biophys. Res. Commun.83:991-997.

3. Carter, A. P., W. M. Clemons, D. E. Brodersen, R. J. Morgan-Warren, B. T. Wimberly, and V. Ramakrishnan. 2000. Functional insights from the structure of the 30S ribosomal subunit and its interactions with antibiotics. Nature407:340-348.

4. Cousin, M. A., D. Lando, T. Ojasoo, and J. P. Raynaud. 1977. Stability of 70S ribosomes in relation to misreading and antibacterial activity of aminoglycosides. Biochimie59:59-63.

5. Dallas, A., and H. F. Noller. 2001. Interaction of translation initiation factor 3 with the 30S ribosomal subunit. Mol. Cell8:855-864.

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