Affiliation:
1. School of Public Health, Division of Infectious Diseases, University of California at Berkeley, Berkeley, California 94720
Abstract
ABSTRACT
Susceptibility of six isolates of
Mycobacterium tuberculosis
(CB3.3, CDC1551, RJ2E, C.C.13, H37Rv, and H37Ra) and two isolates of
Mycobacterium bovis
(Ravenel and BCG) to reactive oxygen intermediates (ROI) and reactive nitrogen intermediates (RNI) was determined by standard in vitro survival assays. After 21 days of incubation, the survival of most strains exposed to either acidified sodium nitrite (ASN) or hydrogen peroxide (H
2
O
2
) was significantly lower than the same strains unexposed to these RNI or ROI products. However, after 50 days of incubation, these differences in susceptibility became less apparent for strains exposed to ASN but not for strains exposed to H
2
O
2
. The recovery of these strains after exposure to RNI suggests that the effect of RNI on
M. tuberculosis
is bacteriostatic. The in vitro concentrations of ROI and RNI used in these assays were higher than those expected in vivo. These observations suggest that, in vivo, RNI expression at physiologically achievable concentrations may keep
M. tuberculosis
from proliferating but that removal of RNI may allow the organisms to proliferate. Furthermore, the ability of some
M. tuberculosis
strains to cause rapidly progressive disease may relate to their intrinsic levels of RNI and ROI resistance.
Publisher
American Society for Microbiology
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