Direct PCR Detection of Burkholderia cepacia Complex and Identification of Its Genomovars by Using Sputum as Source of DNA-Reference-Cited by-同舟云学术

Direct PCR Detection of Burkholderia cepacia Complex and Identification of Its Genomovars by Using Sputum as Source of DNA

Published:2002-09 Issue:9 Volume:40 Page:3485-3488
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Dřevínek Pavel¹,Hrbáčková Hana²,Cinek Ondřej¹,Bartošová Jana¹,Nyč Otakar²,Nemec Alexandr³,Pohunek Petr¹

Affiliation:

1. 2nd Department of Pediatrics, 2nd Medical School of Charles University

2. Department of Medical Microbiology, Motol University Hospital

3. National Institute of Public Health, Prague, Czech Republic

Abstract

ABSTRACT We developed a nested PCR assay that detects the recA gene of the Burkholderia cepacia complex in sputum. The product of the first PCR round is also used to identify the genomovar of the pathogen. The protocol achieves high sensitivity and specificity with simple interpretation of genomovar status.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.40.9.3485-3488.2002

Reference22 articles.

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