Comparison of Serological Detection Methods for Diagnosis of Ehrlichia canis Infections in Dogs

Author:

Bélanger Myriam1,Sorenson Heather L.2,France Michelle K.2,Bowie Michael V.1,Barbet Anthony F.1,Breitschwerdt Edward B.3,Alleman A. Rick2

Affiliation:

1. Departments of Pathobiology

2. Physiological Sciences, College of Veterinary Medicine, University of Florida, Gainesville, Florida 32610

3. Department of Companion Animal and Special Species Medicine, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606

Abstract

ABSTRACT We determined the value of four serological assays for the diagnosis of canine monocytic ehrlichiosis by comparing them to the indirect fluorescent-antibody assay “gold standard.” The specificity of Dip-S-Ticks was significantly lower than that of all of the other tests evaluated. The sensitivity of Dip-S-Ticks was significantly higher than that of Snap3Dx or the Snap Canine Combo. The sensitivity of the rMAP2 enzyme-linked immunosorbent assay (ELISA) was significantly higher than that of the Snap Canine Combo. The accuracy levels of the rMAP2 ELISA, Snap3Dx, Dip-S-Ticks, and Snap Canine Combo were 97.0, 89.8, 85.1, and 82.9%, respectively.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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