Affiliation:
1. Department of Microbiology, College of Medicine, University of Utah, Salt Lake City, Utah
Abstract
Martin, William
J. (University of Utah, Salt Lake City),
and Stanley Marcus
. Detoxified bacterial endotoxins. I. Preparation and biological properties of an acetylated crude endotoxin from
Salmonella typhimurium
. J. Bacteriol.
91:
1453–1459. 1966.—Acetylation of a crude endotoxin prepared by the Roschka-Edwards (RE) procedure from a strain of
Salmonella typhimurium
yields a product with reduced pyrogenicity in rabbits as well as one which is nontoxic in mice. A reduction in pyrogenicity of approximately 100 times was noted with this acetylated crude endotoxin when compared with the parent RE preparation. A comparison was made of immunogenicity with mice of Boivin, RE, and acetylated Roschka-Edwards (Acet-RE) preparations with a heat-killed, phenol-preserved (HP) vaccine prepared from the same strain of
S. typhimurium
. Less than pyrogenic doses of all vaccines were not protective. The least pyrogenic preparation (Acet-RE) was immunogenically effective in about five times the minimal pyrogenic dose. The data suggest that the Acet-RE preparation should be considered further in the search for enteric fever vaccines with lowered potential for undesirable systemic responses.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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