Clinical Performance of Three Commercial Molecular Diagnostic Assays for the Detection of Fluoroquinolone Resistance-Associated Mutations in Mycoplasma genitalium-Reference-Cited by-同舟云学术

Clinical Performance of Three Commercial Molecular Diagnostic Assays for the Detection of Fluoroquinolone Resistance-Associated Mutations in Mycoplasma genitalium

Published:2022-12-21 Issue:12 Volume:60 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Gardette Marie¹,Hénin Nadège²,Le Roy Chloé²,Guiraud Jennifer¹²,Touati Arabella¹,Bébéar Cécile¹²,Pereyre Sabine¹²^ORCID

Affiliation:

1. CHU Bordeaux, Bacteriology Department, National Reference Center for Bacterial Sexually Transmitted Infections, Bordeaux, France

2. University of Bordeaux, CNRS, Fundamental Microbiology and Pathogenicity, UMR 5234, Bordeaux, France

Abstract

The high prevalence of macrolide resistance in Mycoplasma genitalium results in an increased reliance on moxifloxacin, the second-line treatment; however, moxifloxacin resistance has also emerged. Because assays that can detect fluoroquinolone resistance-associated mutations will be useful for the management of macrolide-resistant M. genitalium infections, we evaluated the performance of three commercial assays (the Allplex MG & MoxiR Assay [Seegene], LightMix Modular parC kit [TIBMOLBIOL], and MGMO qPCR [NYtor) in comparison with parC gene Sanger sequencing used as the reference.

Funder

French National Reference Center for Bacterial Sexually Transmitted Infections

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.01135-22

Reference27 articles.

1. Launch of the BASHH guideline for the management of M. genitalium in adults

2. Sexually Transmitted Infections Treatment Guidelines, 2021

3. 2021 European guideline on the management of Mycoplasma genitalium infections

4. Prevalence of mutations associated with resistance to macrolides and fluoroquinolones in Mycoplasma genitalium: a systematic review and meta-analysis

5. Mycoplasma genitalium and antimicrobial resistance in Europe: a comprehensive review

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1. Treatment of bacterial sexually transmitted infections in Europe: gonorrhoea, Mycoplasma genitalium, and syphilis;The Lancet Regional Health - Europe;2023-11

2. Evaluation of TIB Molbiol LightMix® assays for detection of Mycoplasma genitalium and key resistance mutations for macrolides and fluoroquinolones;Letters in Applied Microbiology;2023-10-25

3. Detection of macrolide and fluoroquinolone resistance-associated 23S rRNA and parC mutations in Mycoplasma genitalium by nested real-time PCR;Frontiers in Cellular and Infection Microbiology;2023-10-20