Comparison of Blood Collected in Acid-Citrate-Dextrose and EDTA for Use in Human Immunodeficiency Virus Peripheral Blood Mononuclear Cell Cultures-Reference-Cited by-同舟云学术

Comparison of Blood Collected in Acid-Citrate-Dextrose and EDTA for Use in Human Immunodeficiency Virus Peripheral Blood Mononuclear Cell Cultures

Published:2000-02 Issue:2 Volume:38 Page:858-860
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Fiscus Susan A.¹,Chakraborty Hrishikesh²,Shepard Robin¹,Goodman Melissa¹

Affiliation:

1. Department of Microbiology & Immunology1 and

2. Department of Biostatistics,2 University of North Carolina at Chapel Hill, Chapel Hill, North Carolina

Abstract

ABSTRACT Paired blood samples collected in acid-citrate-dextrose and EDTA were compared for human immunodeficiency virus (HIV) infectivity on the day of collection or after 1 day of storage at room temperature. No significant differences between the anticoagulants were observed. Culture positivity was significantly associated with HIV RNA viral loads for both anticoagulants.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.38.2.858-860.2000

Reference12 articles.

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2. Optimal conditions for recovery of the human immunodeficiency virus from peripheral blood mononuclear cells

3. Optimization of Specimen-Handling Procedures for Accurate Quantitation of Levels of Human Immunodeficiency Virus RNA in Plasma by Reverse Transcriptase PCR

4. Sensitive microculture method for isolation of human immunodeficiency virus type 1 from blood leukocytes

5. HIV-1 isolation from small amounts of whole blood: a technical evaluation;Fiore J. R.;Microbiologica,1992

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