Comparative Study for Determination of Mycobacterium tuberculosis Susceptibility to First- and Second-Line Antituberculosis Drugs by the Etest Using 7H11, Blood, and Chocolate Agar-Reference-Cited by-同舟云学术

Comparative Study for Determination of Mycobacterium tuberculosis Susceptibility to First- and Second-Line Antituberculosis Drugs by the Etest Using 7H11, Blood, and Chocolate Agar

Published:2008-12 Issue:12 Volume:46 Page:4095-4098
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Coban Ahmet Yilmaz¹,Bilgin Kemal¹,Uzun Meltem²,Akgunes Alper¹³,Yusof Anne⁴,Durupinar Belma¹

Affiliation:

1. Ondokuz Mayis University Medical School, Department of Microbiology and Clinical Microbiology, Samsun, Turkey

2. Istanbul University, Istanbul Medical School, Department of Microbiology and Clinical Microbiology, Istanbul, Turkey

3. Chest Disease Hospital, Mycobacteriology Laboratory, Samsun, Turkey

4. AB Biodisk Research Laboratories, Dalvagen 10, 169 56 Solna, Sweden

Abstract

ABSTRACT We investigated the performance of blood and chocolate agar as alternatives to Middlebrook 7H11 agar for testing the susceptibility of Mycobacterium tuberculosis to first-and second-line drugs by the Etest method. A total of 39 strains of M. tuberculosis including 22 multidrug-resistant M. tuberculosis strains and 17 susceptible strains were tested. In conclusion, our results showed that chocolate agar gave insufficient growth, needing up to 21 days of incubation, while results on blood agar were comparable to those on Middlebrook 7H11 agar and can be further explored as an alternative for Etest-based susceptibility testing of M. tuberculosis .

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01104-08

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