Application of the peroxidase-antiperoxidase immunoassay to the identification of Salmonellae from pure culture and animal tissue

Abstract

The peroxidase-antiperoxidase immunoassay was developed by using selected Salmonella serotypes to evaluate its potential for use in diagnostic bacteriology. S. choleraesuis var, kunzendorf, S. dublin, and S. typhimurium were the test organisms. Strong specific staining with corresponding antiserum was achieved with smears of each Salmonella serotype on microscope slides from formalinized cell suspensions, live broth cultures of clinical isolates, and tissue suspensions from the livers and spleens of experimentally infected mice. In addition, S. choleraesuis var. kunzendorf was detected in Formalin-fixed and fresh frozen tissues from experimentally infected pigs. The results of this study indicate that the peroxidase-antiperoxidase assay is well-suited for the rapid identification of Salmonella from pure cultures and that the technique can be useful in research for the detection of this pathogen in histological sections.