Characterization of the Deoxyribonucleic Acid of Various Strains of Halophilic Bacteria-Reference-Cited by-同舟云学术

Characterization of the Deoxyribonucleic Acid of Various Strains of Halophilic Bacteria

Published:1969-07 Issue:1 Volume:99 Page:248-254
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Moore Richard L.¹,McCarthy Brian J.¹

Affiliation:

1. Departments of Microbiology and Genetics, University of Washington, Seattle, Washington 98105

Abstract

Bacteria classified as extreme halophiles, in the genera Halobacterium and Halococcus , contain deoxyribonucleic acid (DNA) which displays two components in a CsCl equilibrium density gradient. The base composition of the major DNA component ranges from 66 to 68% guanine plus cytosine (GC), whereas that of the satellite DNA comprising some 11 to 36% of the total, is between 57 and 60% GC. Purification of the bacterial cells in a CsCl density gradient and other more conventional strain purification procedures both indicated that the presence of the satellite DNA component is not a result of mixed cultures.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.99.1.248-254.1969

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