Affiliation:
1. Department of Microbiology, University of Kansas, Lawrence, Kansas 66044
Abstract
Crude extracts of
Desulfovibrio vulgaris
reduced sulfite to sulfide. Ammonium sulfate fractionation of crude extracts separated a thiosulfate-forming system from sulfite- and thiosulfate-reductase activities. Further purification by sucrose density centrifugation separated the thiosulfate-forming system into two components, both of which were required for the reaction. In addition to these two components, cytochrome
c
3
, ferredoxin, and hydrogenase were required to form thiosulfate from sulfite. By absorption spectra and from the effect of
p
H and substrate concentration, the ionic species acting as the substrate for thiosulfate-formation was concluded to be bisulfite.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference20 articles.
1. The participation of a ferredoxin of Clostridium nigrificans in sulfite reduction;Akagi J. M.;Biochem. Biophys. Res. Commun.,1965
2. Electron carriers for the phosphoroclastic reaction of Desulfovibrio desulfuricans;Akagi J. M.;J. Biol. Chem.,1967
3. Studies on thermophilic sulfate-reducing bacteria. II. Hydrogenase activity of Clostridium nigrificans;Akagi J. M.;J. Bacteriol.,1961
4. Studies on thermophilic sulfate-reducing bacteria. 111. Adenosine triphosphatesulfurylase of Clostridium nigrificans and Desulfovibrio desulfuricans;Akagi J. M.;J. Bacteriol.,1962
5. The equilibria in aqueous solutions of alkali metal bisulfites;Baly E. C. E.;J. Chem. Soc. (London),1922
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