Method for detecting production of zearalenone, zearalenol, T-2 toxin, and deoxynivalenol by Fusarium isolates

Author:

Richardson K E,Hagler W M,Hamilton P B

Abstract

Three methods for detecting toxigenic fusaria in culture were compared by using known producers of zearalenone, zearalenol, T-2 toxin, and deoxynivalenol. Moist, autoclaved rice cultures of known toxigenic isolates grown in 20-ml tubes yielded oily extracts containing compounds which interfered with qualitative and quantitative analysis for the mycotoxins. Vermiculite moistened with nutrient broth in 20-ml tubes yielded a much cleaner extract. Growing the fungi on a liquid medium required a shorter incubation period, but yields of T-2 toxin and deoxynivalenol were low and variable, and the method required greater space in the incubator. Screening of the extracts by thin-layer chromatography with colorimetric spray reagents to detect the presence of these toxins permitted reduction in the number of extracts quantified by the more lengthy gas-liquid chromatographic method. Culturing in nutrient broth on vermiculite in tubes coupled to a qualitative screen before quantitation proved to be a convenient, inexpensive, and relatively rapid method that enabled reliable screening of a large number of Fusarium isolates for toxin production as compared with prior methods.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference24 articles.

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1. Occurrence of Zearalenone, Zearalenol and Deoxynivalenol in Some Market Samples of Dried Vegetables in J&K State;Proceedings of the National Academy of Sciences, India Section B: Biological Sciences;2012-06-04

2. Toxicity and trichothecene production byFusarium acuminatum subsp.acuminatum andFusarium acuminatum subsp.armeniacum;Natural Toxins;1993

3. Chapter 9 Gas Chromatography of Mycotoxins;Chromatography of Mycotoxins - Techniques and Applications;1993

4. Chapter 7 Thin-Layer Chromatography of Mycotoxins;Chromatography of Mycotoxins - Techniques and Applications;1993

5. Determination of α- and β-zearalenol and zearalenone in cereals by gas chromatography with ion-trap detection;Journal of Chromatography A;1992-03

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