In Vitro Evaluation of Biofilm Dispersal as a Therapeutic Strategy To Restore Antimicrobial Efficacy-Reference-Cited by-同舟云学术

In Vitro Evaluation of Biofilm Dispersal as a Therapeutic Strategy To Restore Antimicrobial Efficacy

Published:2017-10 Issue:10 Volume:61 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Roizman Dan¹²,Vidaillac Celine¹,Givskov Michael¹³,Yang Liang¹⁴^ORCID

Affiliation:

1. Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore

2. Interdisciplinary Graduate School, Nanyang Technological University, Singapore

3. Costerton Biofilm Center, Department of Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark

4. School of Biological Sciences, Nanyang Technological University, Singapore

Abstract

ABSTRACT As a proof-of-concept study, the direct impact of biofilm dispersal on the in vitro efficacy of imipenem and tobramycin was evaluated against 3-day-old biofilms of Pseudomonas aeruginosa . Arabinose induction of biofilm dispersal via activation of the phosphodiesterase YhjH in the P. aeruginosa engineered strain PAO1/p BAD - yhjH resulted in increased antimicrobial efficacy and synergy of the imipenem-tobramycin combination. These results support the use of biofilm dispersal to enhance antimicrobial efficacy in the treatment of biofilm-associated infections, representing a promising therapeutic strategy.

Funder

National Research Foundation Singapore

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.01088-17

Reference15 articles.

1. ESCMID∗ guideline for the diagnosis and treatment of biofilm infections 2014

2. Bacterial Biofilms: Development, Dispersal, and Therapeutic Strategies in the Dawn of the Postantibiotic Era

3. In vitro and in vivo generation and characterization of Pseudomonas aeruginosa biofilm–dispersed cells via c-di-GMP manipulation