Isotype antibody response in cows to Streptococcus agalactiae group B polysaccharide-ovalbumin conjugate

Abstract

Adult dairy cows were immunized with group B antigen (GBA) of Streptococcus agalactiae or GBA coupled to ovalbumin, both emulsified in incomplete Freund adjuvant, and their sera were examined by an enzyme-linked immunosorbent assay measuring bovine immunoglobulin isotypes (immunoglobulin G1 [IgG1], IgG2, and IgM) specific for GBA. All of the cows possessed naturally acquired antibodies against GBA, which implied that primary antibody responses could not be studied. At the highest dose tested (200 micrograms), free GBA elicited a slight increase in antibody titers only in the IgM isotype, to which most of the naturally acquired antibodies to GBA belonged. A second administration of antigen was not more effective. The conjugate was able to induce a strong humoral response against GBA, particularly in the IgG1 and IgG2 subisotypes, and a second injection of the conjugate induced a doubling of the peak antibody titers. Therefore, conjugation of GBA to a protein carrier markedly improved the antibody response, which showed the main characteristics of T-cell dependency. The opsonic activity of serum against an unencapsulated strain of S. agalactiae was reinforced by the immunization with the conjugate.