Use of Hemo-De to eliminate toxic agents used for concentration and trichrome staining of intestinal parasites

Author:

Aldeen W E1,Hale D1

Affiliation:

1. Associated Regional and University Pathologists, Inc., Salt Lake City, Utah 84108.

Abstract

In a blind comparison, 465 randomly collected clinical fecal specimens were examined. Hemo-De was found to be an excellent replacement for ethyl acetate in the concentration procedure and for carbol-xylene and xylene in the trichrome staining procedure. Elimination of toxic reagents, combined with its lower cost, makes Hemo-De the preferred choice in routine parasitology examinations.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference14 articles.

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2. Comparison of clinical results for the use of ethyl acetate and diethyl ether in the Formalin-ether sedimentation technique performed on polyvinyl alcohol-preserved specimens;Garcia L. S.;J. Clin. Microbiol.,1981

3. Use of terpene-based products, with and without anhydrous alcohol, as clearing agents in trichrome staining technique for intestinal protozoa;Gubash S. M.;J. Clin. Microbiol.,1990

4. Melvin D. M. and M. M. Brooke. 1982. Laboratory procedures for the diagnosis of intestinal parasites. U.S. Department of Health and Human Services publication no. (CDC) 82-8282. Centers for Diseases Control Atlanta.

5. Modified trichrome staining technique with a xylene substitute;Neimeister R.;J. Clin. Microbiol.,1985

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