Nitroaromatics Are Substrates for the TOL Plasmid Upper-Pathway Enzymes-Reference-Cited by-同舟云学术

Nitroaromatics Are Substrates for the TOL Plasmid Upper-Pathway Enzymes

Published:1992-01 Issue:1 Volume:58 Page:415-417
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Delgado Asunción¹,Wubbolts Marcel G.¹,Abril María-Angeles¹,Ramos Juan L.¹

Affiliation:

1. Departamento de Bioquímica Vegetal, Consejo Superior de Investigaciones Científicas, Estación Experimental del Zaidín, Apto. 419, 18080 Granada, Spain, and Department of Biochemistry, University of Groningen, Groningen, The Netherlands2

Abstract

Expression of the xylMA genes encoding for toluene monoxygenase from the lactose promoter in a broad-host-range plasmid allows the oxidation of toluene and m - and p -nitrotoluene to their corresponding benzyl alcohols and benzaldehydes in Pseudomonas putida and Escherichia coli . Benzyl alcohols accumulate until reaching a concentration of about 80 μM, while benzaldehydes accumulate steadily with time for at least 24 h. TOL-encoded benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase recognize m - and p -nitro-substituted compounds as substrates. In contrast, the XylR protein, which regulates the TOL plasmid-encoded upper-pathway operon, does not recognize nitro-substituted toluenes as effectors.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.58.1.415-417.1992

Reference9 articles.

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