Diversity among Streptomyces Strains Causing Potato Scab

Abstract

Eighty Streptomyces isolates, including 35 potato scab-inducing strains and 12 reference strains of Streptomyces scabies , were physiologically characterized by a total of 329 miniaturized tests. Overall similarities of all strains were determined by numerical taxonomy, with the unweighted average linkage (UPGMA) algorithm and simple matching ( S sm ) and Jaccard ( S j ) coefficients used as measures for similarity. Three cluster groups (A to C) were defined at a similarity level of 80.1% ( S sm ); these groups contained 14 clusters and 24 unclustered strains defined at a similarity level of 86.5% ( S sm ). Cluster group A contained strains phenotypically related to S. griseus or S. exfoliatus , whereas cluster group B contained strains which were phenotypically related to S. violaceus or S. rochei . The majority of the pathogenic isolates and reference strains were assigned to S. violaceus (57%) and S. griseus (22%). A DNA probe derived from the rRNA operon of S. coelicolor IMET 40271 was used to detect restriction fragment length polymorphisms (RELPs) among 40 pathogenic and nonpathogenic Streptomyces isolates. Southern blots revealed a high degree of diversity among the pathogenic strains tested. No significant correlation between numerical classification and RFLP grouping of Streptomyces strains could be revealed. The results obtained suggest that RFLP data are of minor importance in classification of Streptomyces species and that genes for pathogenicity determinants are spread among different Streptomyces species by mobilizable elements.