Abstract
The mechanism by which L-proline triggers germination in Bacillus megaterium QM B1551 spores was investigated. First, brief exposure of spores to L-proline, followed by dilution, was sufficient to trigger germination. Once germination was triggered, the spores continued initiation of germination and did not require high concentrations of L-proline. Triggering of germination was pH and temperature dependent. Second, enzymes for L-proline catabolism were absent in spores, and several non-metabolizable analogs of L-proline were effective trigger compounds. Third, triggering of germination occurred in the presence of inhibitors of proton motive force production, oxygen uptake, and metabolism. Fourth, uptake of L-proline occurred after the triggering of germination. These results argue that neither uptake nor metabolism of L-proline was necessary to trigger germination. Instead, L-proline probably causes a biophysical alteration in the spores that triggers the biochemical changes in germination.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference40 articles.
1. Chemotaxis in bacteria. Annu;Adler J.;Rev. Biochem.,1975
2. Ando Y. 1977. Biochemical mechanisms of ionic germination of spores of Clostridium perfringens type A p. 75-79. In G. Chambliss and J. C. Vary (ed.) Spores VII. American Society for Microbiology Washington D.C.
3. Identity of proline dehydrogenase and A'-pyrroline-5-carboxylic acid dehydrogenase in Clostridium sporogenes;Costilow R. N.;J. Bacteriol.,1978
4. Regulation of proline degradation in Salmonella typhimurium;Dendinger S.;J. Bacteriol.,1970
5. Effect of the proline analogue baikiain on proline metabolism in Salmonella typhimurium;Dendinger S.;J. Bacteriol.,1972
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