Enhancement of Culture of Legionella longbeachae from Respiratory Samples by Use of Immunomagnetic Separation and Antimicrobial Decontamination-Reference-Cited by-同舟云学术

Enhancement of Culture of Legionella longbeachae from Respiratory Samples by Use of Immunomagnetic Separation and Antimicrobial Decontamination

Published:2020-10-21 Issue:11 Volume:58 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Mohammadi Ali¹,Chambers Stephen T.¹²^ORCID,Scott-Thomas Amy¹,Lewis John G.³,Anderson Trevor⁴,Podmore Ros⁴,Williman Jonathan⁵,Murdoch David¹³,Slow Sandy¹

Affiliation:

1. Department of Pathology and Biomedical Science, University of Otago, Christchurch, New Zealand

2. Department of Infectious Diseases, Christchurch Hospital, Christchurch, New Zealand

3. Department of Biochemistry, Canterbury Health Laboratories, Christchurch, New Zealand

4. Department of Microbiology, Canterbury Health Laboratories, Christchurch, New Zealand

5. Department of Population Health, University of Otago, Christchurch, New Zealand

Abstract

Legionella longbeachae is the commonest Legionella species identified in patients with community-acquired pneumonia in New Zealand. Isolation of the organism on culture is the gold standard for the diagnosis of Legionnaires disease, but it has poor sensitivity (40%) compared with quantitative PCR (qPCR). We have developed a selective decontamination process using glycine, vancomycin, polymyxin, and cycloheximide (GVPC) with immunomagnetic separation (IMS) for culturing L. longbeachae .

Funder

Te Whare Wānanga o Otāgo | Division of Health Sciences, University of Otago

Maurice and Phyllis Paykel Trust

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01218-20

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