Affiliation:
1. College of Life Science, Wuhan University, Wuhan 430072, People's Republic of China
Abstract
ABSTRACT
The brown planthopper (
Nilaparvata lugens
Stål), the most destructive pest of rice, has been identified, including biotypes with high virulence towards previously resistant rice varieties. There have also been many reports of a yeast-like symbiont of
N. lugens
, but little is known about the bacterial microbes. In this study, we examined the bacterial microbes in
N. lugens
and identified a total of 18 operational taxonomic units (OTUs) representing four phyla (
Proteobacteria
,
Firmicutes
,
Actinobacteria
, and
Bacteroidetes
) by sequencing and analyzing 16S rRNA gene libraries obtained from three populations of
N. lugens
, which were maintained on the rice varieties TN1, Mudgo, and ASD7. Several of the OTUs were similar to previously reported secondary symbionts of other insects, including an endosymbiont of the psyllid
Glycapsis brimblecombei
, an
Asaia
sp. found in the mosquito
Anopheles stephensi
, and
Wolbachia
, found in the mite
Metaseiulus occidentalis
. However, the species and numbers of the detected OTUs differed substantially among the
N. lugens
populations. Further,
in situ
hybridization analysis using digoxigenin-labeled probes indicated that OTU 1 was located in hypogastrium tissues near the ovipositor and ovary in biotype 1 insects, while OTU 2 was located in the front of the ovipositor sheath in biotype 2 insects. In addition, masses of bacterium-like organisms were observed in the tubes of salivary sheaths in rice plant tissues that the insects had fed upon. The results provide indications of the diversity of the bacterial microbes harbored by the brown planthopper and of possible associations between specific bacterial microbes and biotypes of
N. lugens
.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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