Affiliation:
1. Genomics Research Institute, SRL, Inc., 5-6-50 Shinmachi, Hino-shi, Tokyo 191-0002, Japan
Abstract
ABSTRACT
Phylogenetic analysis of about 200 strains of
Salmonella
,
Shigella
, and
Escherichia coli
was carried out using the nucleotide sequence of the gene for DNA gyrase B (
gyrB
), which was determined by directly sequencing PCR fragments. The results establish a new phylogenetic tree for the classification of
Salmonella
,
Shigella
, and
Escherichia coli
in which
Salmonella
forms a cluster separate from but closely related to
Shigella
and
E. coli.
In comparison with 16S rRNA analysis, the
gyrB
sequences indicated a greater evolutionary divergence for the bacteria. Thus, in screening for the presence of bacteria, the
gyrB
gene might be a useful tool for differentiating between closely related species of bacteria such as
Shigella
spp. and
E. coli
. At present, 16S rRNA sequence analysis is an accurate and rapid method for identifying most unknown bacteria to the genus level because the highly conserved 16S rRNA region is easy to amplify; however, analysis of the more variable
gyrB
sequence region can identify unknown bacteria to the species level. In summary, we have shown that
gyrB
sequence analysis is a useful alternative to 16S rRNA analysis for constructing the phylogenetic relationships of bacteria, in particular for the classification of closely related bacterial species.
Publisher
American Society for Microbiology
Cited by
142 articles.
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