Evaluation of the Phenol Ammonium Sulfate Sedimentation Smear Microscopy Method for Diagnosis of Pulmonary Tuberculosis-Reference-Cited by-同舟云学术

Evaluation of the Phenol Ammonium Sulfate Sedimentation Smear Microscopy Method for Diagnosis of Pulmonary Tuberculosis

Published:2002-08 Issue:8 Volume:40 Page:3017-3020
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Selvakumar N.¹,Rahman Fathima¹,Garg Renu²,Rajasekaran S.³,Mohan Nalini Sunder¹,Thyagarajan K.¹,Sundaram V.¹,Santha T.¹,Frieden Thomas R.²,Narayanan P. R.¹

Affiliation:

1. Tuberculosis Research Centre (ICMR)

2. South East Asia Regional Office, Stop Tuberculosis Unit, World Health Organization, New Delhi, India

3. Government Hospital for Thoracic Medicine, Tambaram Sanatorium, Chennai

Abstract

ABSTRACT We compared the sensitivity and specificity of the phenol ammonium sulfate (PhAS) sediment smear microscopy method for detection of acid-fast bacilli with those of direct smear microscopy, using culture results for Mycobacterium tuberculosis as the “gold standard.” The sensitivities of the PhAS and direct smear methods were 85% (465 of 547) and 83% (454 of 547), respectively, and the specificity of each method was 97%. The PhAS method was better accepted by the laboratory technicians and safer but necessitates an overnight sedimentation, which delays reporting of results until 1 day after sputum collection.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.40.8.3017-3020.2002

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