Serologic and Molecular Detection of Ehrlichia chaffeensis and Anaplasma phagocytophila (Human Granulocytic Ehrlichiosis Agent) in Korean Patients

Author:

Heo Eun-jeong1,Park Jin-ho2,Koo Ja-ryong3,Park Man-suk4,Park Mi-yeoun4,Dumler J. Stephen2,Chae Joon-seok1

Affiliation:

1. Department of Veterinary Internal Medicine and Clinical Pathology, College of Veterinary Medicine, Chonbuk National University, Jeonju, Jeonbuk 561-756

2. Division of Medical Microbiology, Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, Maryland 21287

3. Mol-BioNet Research Institute, Scientec Lab Center Co., Ltd., Daeduk-Gu, Taejon 306-030

4. Division of Rickettsial & Zoonotic Diseases, Department of Microbiology, National Institute of Health, Seoul 122-701, Korea

Abstract

ABSTRACT Sera from 491 Korean patients with acute febrile diseases were tested for Ehrlichia chaffeensis and Anaplasma phagocytophila antibodies by indirect immunofluorescence assay (IFA), Western blotting, and TaqMan real-time PCR. Overall, 0.4% of sera reacted with E. chaffeensis , and 1.8% reacted with A. phagocytophila in IFAs. This is the first report of detection of antibodies to A. phagocytophila and E. chaffeensis in Korea and suggests the presence of A. phagocytophila and E. chaffeensis or antigenically similar species.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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