Affiliation:
1. Biomedical Genomics Center and Department of Veterinary Pathobiology, University of Minnesota, St. Paul, Minnesota
Abstract
ABSTRACT
Repetitive sequence-based PCR (rep-PCR) and amplified fragment length polymorphism (AFLP) were used to characterize a sample of 43 field isolates and 4 attenuated vaccine strains of
Pasteurella multocida
recovered from multiple avian species. Both rep-PCR and AFLP assays were rapid and reproducible, with high indices of discrimination. Concordance analyses of rep-PCR and AFLP with somatic serotyping indicate that, in general, somatic serotyping is a poor indicator of genetic relatedness among isolates of
P. multocida
. In addition, the data provide evidence of host specificity of
P. multocida
clones. Overall, the results of our study indicate that the rep-PCR and AFLP techniques enable rapid fingerprinting of
P. multocida
isolates from multiple avian species and enhance the investigation of fowl cholera outbreaks.
Publisher
American Society for Microbiology
Cited by
34 articles.
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