Validation of Nasopharyngeal Sampling and Culture Techniques for Detection of Streptococcus pneumoniae in Children in Kenya-Reference-Cited by-同舟云学术

Validation of Nasopharyngeal Sampling and Culture Techniques for Detection of Streptococcus pneumoniae in Children in Kenya

Published:2007-10 Issue:10 Volume:45 Page:3408-3410
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Abdullahi Osman¹,Wanjiru Eva¹,Musyimi Robert¹,Glass Nina²,Scott J. Anthony G.¹³

Affiliation:

1. Kenya Medical Research Institute/Wellcome Trust Research Program, Centre for Geographic Medicine Research—Coast, Kilifi, Kenya

2. Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia

3. Nuffield Department of Clinical Medicine, Oxford University, John Radcliffe Hospital, Oxford, United Kingdom

Abstract

ABSTRACT We compared nasopharyngeal swabs against nasal wash cultures for detecting colonizing pneumococci and examined the effect of frozen storage in skim milk-tryptone-glucose-glycerin on culture. Among the 55 children with positive nasal wash cultures, swab cultures were positive for 47 (85%). Of the 96 swabs positive on direct plating, 94 (98%) were positive when recultured after freezing.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01393-07

Reference4 articles.

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3. Hendley, J. O., M. A. Sande, P. M. Stewart, and J. M. Gwaltney, Jr. 1975. Spread of Streptococcus pneumoniae in Families. I. Carriage rates and distribution of types. J. Infect. Dis.132:55-61.

4. O'Brien, K., H. Nohynek, et al. 2003. The pneumococcal vaccine trials carriage working group. Report from a WHO working group: standard method for detecting upper respiratory carriage of Streptococcus pneumoniae. Pediatr. Infect. Dis. J.22:133-140.

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