Comparison of Cultures from Rectoanal-Junction Mucosal Swabs and Feces for Detection of Escherichia coli O157 in Dairy Heifers-Reference-Cited by-同舟云学术

Comparison of Cultures from Rectoanal-Junction Mucosal Swabs and Feces for Detection of Escherichia coli O157 in Dairy Heifers

Published:2006-05 Issue:5 Volume:72 Page:3766-3770
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Davis Margaret A.¹,Rice Daniel H.¹²,Sheng Haiqing¹,Hancock Dale D.²,Besser Thomas E.³,Cobbold Rowland²,Hovde Carolyn J.¹

Affiliation:

1. Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 83844-3052

2. Department of Veterinary Clinical Sciences, Washington State University, Pullman, Washington 99164-6610

3. Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040

Abstract

ABSTRACT Fecal culture for Escherichia coli O157:H7 was compared to rectoanal mucosal swab (RAMS) culture in dairy heifers over a 1-year period. RAMS enrichment culture was as sensitive as fecal culture using immunomagnetic separation (IMS) ( P = 0.98, as determined by a chi-square test). RAMS culture is less costly than fecal IMS culture and can yield quantitative data.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.72.5.3766-3770.2006

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