Metal Reduction and Iron Biomineralization by a Psychrotolerant Fe(III)-Reducing Bacterium, Shewanella sp. Strain PV-4

Author:

Roh Yul12,Gao Haichun13,Vali Hojatollah4,Kennedy David W.5,Yang Zamin K.1,Gao Weimin1,Dohnalkova Alice C.5,Stapleton Raymond D.6,Moon Ji-Won1,Phelps Tommy J.1,Fredrickson James K.5,Zhou Jizhong17

Affiliation:

1. Oak Ridge National Laboratory, Oak Ridge, Tennessee

2. Chonnam National University, Gwangju, South Korea

3. Center for Microbial Ecology, Michigan State University, East Lansing, Michigan

4. McGill University, Montreal, Quebec, Canada

5. Pacific Northwest National Laboratory, Richland, Washington

6. Merck & Co., Inc., Elkton, Virginia

7. Institute for Environmental Genomics, Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73019

Abstract

ABSTRACT A marine psychrotolerant, dissimilatory Fe(III)-reducing bacterium, Shewanella sp. strain PV-4, from the microbial mat at a hydrothermal vent of Loihi Seamount in the Pacific Ocean has been further characterized, with emphases on metal reduction and iron biomineralization. The strain is able to reduce metals such as Fe(III), Co(III), Cr(VI), Mn(IV), and U(VI) as electron acceptors while using lactate, formate, pyruvate, or hydrogen as an electron donor. Growth during iron reduction occurred over the pH range of 7.0 to 8.9, a sodium chloride range of 0.05 to 5%, and a temperature range of 0 to 37°C, with an optimum growth temperature of 18°C. Unlike mesophilic dissimilatory Fe(III)-reducing bacteria, which produce mostly superparamagnetic magnetite (<35 nm), this psychrotolerant bacterium produces well-formed single-domain magnetite (>35 nm) at temperatures from 18 to 37°C. The genome size of this strain is about 4.5 Mb. Strain PV-4 is sensitive to a variety of commonly used antibiotics except ampicillin and can acquire exogenous DNA (plasmid pCM157) through conjugation.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference43 articles.

1. Barry, A. L., and C. Thornsberry. 1991. Susceptibility testing: diffusion test procedures, p. 1117-1125. In A. Balows, J. Hausler, K. Herrmann, H. Isenberg, and H. Shadomy (ed.), Manual of clinical microbiology, 5th ed. American Society for Microbiology, Washington, D.C.

2. Bazylinski, D. A., and B. M. Moskowitz. 1997. Microbial biomineralization of magnetic iron minerals: microbiology, magnetism and environmental significance. Rev. Mineral. Geochem.35:181-224.

3. Biogeochemical Conditions Favoring Magnetite Formation during Anaerobic Iron Reduction

4. Bowman, J. P., S. A. McCammon, D. S. Nichols, J. H. Skerratt, S. M. Rea, P. D. Nichols, and T. A. McMeekin. 1997. Shewanella gelidimarina sp. nov. and Shewanella frigidimarina sp. nov., novel Antarctic species with the ability to produce eicosapentaenoic acid (20:5 omega 3) and grow anaerobically by dissimilatory Fe(III) reduction. Int. J. Syst. Bacteriol.47:1040-1047.

5. Bozal, N., M. J. Montes, E. Tudela, F. Jimenez, and J. Guinea. 2002. Shewanella frigidimarina and Shewanella livingstonensis sp. nov. isolated from Antarctic coastal areas. Int. J. Syst. Evol. Microbiol.52:195-205.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3