Cryo-EM Structures of CusA Reveal a Mechanism of Metal-Ion Export-Reference-Cited by-同舟云学术

Cryo-EM Structures of CusA Reveal a Mechanism of Metal-Ion Export

Published:2021-04-27 Issue:2 Volume:12 Page:
ISSN:2150-7511
Container-title:mBio
language:en
Short-container-title:mBio

Author:

Moseng Mitchell A.¹,Lyu Meinan¹,Pipatpolkai Tanadet²^ORCID,Glaza Przemyslaw¹,Emerson Corey C.¹,Stewart Phoebe L.¹^ORCID,Stansfeld Phillip J.³^ORCID,Yu Edward W.¹^ORCID

Affiliation:

1. Department of Pharmacology, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA

2. Department of Biochemistry, University of Oxford, Oxford, United Kingdom

3. School of Life Sciences & Department of Chemistry, University of Warwick, Coventry, United Kingdom

Abstract

The bacterial RND superfamily of efflux pumps mediate resistance to a variety of biocides, including Cu(I) and Ag(I) ions. Here we report four cryo-EM structures of the trimeric CusA pump in the presence of Cu(I). Combined with MD simulations, our data indicate that each CusA protomer within the trimer recognizes and extrudes Cu(I) independently.

Funder

HHS | NIH | Office of Extramural Research, National Institutes of Health

Wellcome

UKRI | Medical Research Council

UKRI | Biotechnology and Biological Sciences Research Council

Publisher

American Society for Microbiology

Subject

Virology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mBio.00452-21

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