Cultivation of mycoplasmas in a modified tissue culture medium

Author:

Gabridge M G,Singer S E,Esposito R A

Abstract

A new medium, which contained a chemically defined tissue culture base ("medium 199"), was developed for the cultivation of mycoplasmas. When supplemented with albumin, glucose, serum, and yeast extract, the new medium adequately supported the growth of Mycoplasma and Acholeplasma species.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference19 articles.

1. The influence of pH of the culture medium on the sensitivity of Mycoplasma gallisepticum antigens for use in certain serological tests;Bradbury J. M.;J. Hyg.,1971

2. Nuclease activities of Mycoplasma gallisepticum as a function of culture age in different media;Cowan B. S.;J. Bacteriol.,1972

3. Cultivation of mycoplasmas on cellulose ester substrates;Gabridge M. G.;App. Microbiol.,1973

4. Oxygen consumption by trachea organ cultures infected with Mycoplasma pneumoniae;Gabridge M. G.;Infect. Immun.,1975

5. Quantitative reduction of 2,3,5-triphenyl tetrazolium chloride by hamster trachea cultures: effects of Mycoplasma pneumoniae cells and membranes;Gabridge M. G.;Infect. Immun.,1976

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