MRSA Isolates from Patients with Persistent Bacteremia Generate Nonstable Small Colony Variants In Vitro within Macrophages and Endothelial Cells during Prolonged Vancomycin Exposure

Author:

Fauerharmel-Nunes Thiago1,Flannagan Ronald S.1ORCID,Goncheva Mariya I.1ORCID,Bayer Arnold S.234,Fowler Vance G.5,Chan Liana C.2346,Yeaman Michael R.2346,Xiong Yan Q.234,Heinrichs David E.1ORCID

Affiliation:

1. Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada

2. The Lundquist Institute for Biomedical Innovation, Harbor-UCLA Medical Center, Torrance, California, USA

3. David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California, USA

4. Division of Infectious Diseases, Department of Medicine, Harbor-UCLA Medical Center, Torrance, California, USA

5. Duke University Medical Center, Durham, North Carolina, USA

6. Division of Molecular Medicine, Department of Medicine, Harbor-UCLA Medical Center, Torrance, California, USA

Abstract

Staphylococcus aureus (especially methicillin-resistant S. aureus [MRSA]) is frequently associated with persistent bacteremia (PB) during vancomycin therapy despite consistent susceptibility in vitro . Strategic comparisons of PB strains versus those from vancomycin-resolving bacteremia (RB) would yield important mechanistic insights into PB outcomes.

Funder

Gouvernement du Canada | Canadian Institutes of Health Research

HHS | NIH | National Institute of Allergy and Infectious Diseases

U.S. Department of Defense

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

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