Affiliation:
1. Department of Human Microbiology, Sackler School of Medicine, Tel-Aviv University, Ramat-Aviv 69978, Tel-Aviv, Israel
Abstract
ABSTRACT
The role of secreted proteases in the virulence of the pathogenic fungus
Aspergillus fumigatus
remains controversial. Recently, the
Aspergillus niger
transcription factor PrtT was shown to control the expression of multiple secreted proteases. In this work, the gene which encodes the PrtT homolog in
A. fumigatus
was cloned and its function analyzed using a deletion mutant strain. Deletion of
A. fumigatus prtT
resulted in the loss of secreted protease activity. The expression of six secreted proteases (ALP, MEP, Dpp4, CpdS, AFUA_2G17330, and AFUA_7G06220) was markedly reduced. Culture filtrates from the
prtT
deletion strain exhibited reduced killing of lung epithelial cells and lysis of erythrocytes. However, the
prtT
deletion strain did not exhibit altered virulence in lung-infected mice. These results suggest that PrtT is not a significant virulence factor in
A. fumigatus
.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
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