Rapid and Highly Sensitive Pathotyping of Avian Influenza A H5N1 Virus by Using Real-Time Reverse Transcription-PCR-Reference-Cited by-同舟云学术

Rapid and Highly Sensitive Pathotyping of Avian Influenza A H5N1 Virus by Using Real-Time Reverse Transcription-PCR

Published:2007-02 Issue:2 Volume:45 Page:600-603
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Hoffmann Bernd¹,Harder Timm¹,Starick Elke¹,Depner Klaus¹,Werner Ortrud¹,Beer Martin¹

Affiliation:

1. Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, D-17493 Greifswald-Insel Riems, Germany

Abstract

ABSTRACT Rapid typing of the pathogenicity of avian influenza A viruses (AIV) of subtypes H5 and H7 is crucial to initiate adequate protective measures preventing the spread of highly pathogenic AIV (HPAIV). Here, a new real-time reverse transcription-PCR assay which enables sensitive and specific detection and cleavage site analysis of HPAIV H5N1 of the Qinghai lineage is described.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01681-06

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