Effective Detection of Toxigenic Clostridium difficile by a Two-Step Algorithm Including Tests for Antigen and Cytotoxin-Reference-Cited by-同舟云学术

Effective Detection of Toxigenic Clostridium difficile by a Two-Step Algorithm Including Tests for Antigen and Cytotoxin

Published:2006-03 Issue:3 Volume:44 Page:1145-1149
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Ticehurst John R.¹²³,Aird Deborah Z.¹,Dam Lisa M.²,Borek Anita P.¹,Hargrove John T.²,Carroll Karen C.¹³

Affiliation:

1. Division of Medical Microbiology, Johns Hopkins Hospital

2. Clinical Laboratories, Johns Hopkins Bayview Medical Center

3. Departments of Pathology and Medicine, Johns Hopkins Medicine, Baltimore, Maryland

Abstract

ABSTRACT We evaluated a two-step algorithm for detecting toxigenic Clostridium difficile : an enzyme immunoassay for glutamate dehydrogenase antigen (Ag-EIA) and then, for antigen-positive specimens, a concurrent cell culture cytotoxicity neutralization assay (CCNA). Antigen-negative results were ≥99% predictive of CCNA negativity. Because the Ag-EIA reduced cell culture workload by ≈75 to 80% and two-step testing was complete in ≤3 days, we decided that this algorithm would be effective. Over 6 months, our laboratories' expenses were US$143,000 less than if CCNA alone had been performed on all 5,887 specimens.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.44.3.1145-1149.2006

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