Consecutive Use of Two Multiplex PCR-Based Assays for Simultaneous Identification and Determination of Capsular Status of Nine Common Neisseria meningitidis Serogroups Associated with Invasive Disease-Reference-Cited by-同舟云学术

Consecutive Use of Two Multiplex PCR-Based Assays for Simultaneous Identification and Determination of Capsular Status of Nine Common Neisseria meningitidis Serogroups Associated with Invasive Disease

Published:2006-03 Issue:3 Volume:44 Page:1127-1131
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Bennett Désirée E.¹,Cafferkey Mary T.¹²

Affiliation:

1. Epidemiology and Molecular Biology Unit, The Children's University Hospital, Dublin

2. Department of Clinical Microbiology, Royal College of Surgeons in Ireland, York Street, Dublin, Ireland

Abstract

ABSTRACT We developed two Neisseria meningitidis multiplex PCR assays to be used consecutively that allow determination of the serogroup and capsular status of serogroup A, B, C, 29E, W135, X, and Y cnl-3 / cnl-1 -like-containing N. meningitidis isolates by direct analysis of the amplicon size. These assays offer a rapid and simple method of serogrouping N. meningitidis .

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.44.3.1127-1131.2006

Reference36 articles.

1. PCR-Based Assay for Detection of Neisseria meningitidis Capsular Serogroups 29E, X, and Z

2. Borrow, R., H. Claus, M. Guiver, L. Smart, D. Jones, E. Kaczmarski, M. Frosch, and A. Fox. 1997. Non-culture diagnosis and serogroup determination of meningococcal B and C infection by a sialyltransferase (siaD) PCR ELISA. Epidemiol. Infect.118:111-117.

3. Cartwright, K. 2001. Epidemiology, surveillance, and population biology: carriage studies. Methods Mol. Med.67:293-311.

4. Clonal diversity of Neisseria meningitidis from a population of asymptomatic carriers

5. Claus, H., M. Maiden, R. Maag, M. Frosch, and U. Vogel. 2002. Many carried meningococci lack the genes required for capsule synthesis and transport. Microbiology148:1813-1819.

Cited by 24 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Ultra-efficient multiple cross displacement amplification-lateral flow biosensor (MCDA-LFB) for serogroup identification of prevalent Neisseria meningitidis;Analytical Biochemistry;2022-10

2. Risk factors for carriage of meningococcus in third-level students in Ireland: an unsupervised machine learning approach;Human Vaccines & Immunotherapeutics;2021-06-24

3. Genomic Surveillance of a Globally Circulating Distinct Group W Clonal Complex 11 Meningococcal Variant, New Zealand, 2013–2018;Emerging Infectious Diseases;2021-04

4. Antibiotic susceptibility and molecular analysis of invasive Neisseria meningitidis recovered in the Republic of Ireland, 1996 to 2016;European Journal of Clinical Microbiology & Infectious Diseases;2021-01-05

5. Diversity of meningococci associated with invasive meningococcal disease in the Republic of Ireland over a 19 year period, 1996-2015;PLOS ONE;2020-02-13