Candida glabrata Transcription Factor Rpn4 Mediates Fluconazole Resistance through Regulation of Ergosterol Biosynthesis and Plasma Membrane Permeability-Reference-Cited by-同舟云学术

Candida glabrata Transcription Factor Rpn4 Mediates Fluconazole Resistance through Regulation of Ergosterol Biosynthesis and Plasma Membrane Permeability

Published:2020-08-20 Issue:9 Volume:64 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Pais Pedro¹²,Califórnia Raquel¹²,Galocha Mónica¹²,Viana Romeu¹²,Ola Mihaela³,Cavalheiro Mafalda¹²,Takahashi-Nakaguchi Azusa⁴,Chibana Hiroji⁴,Butler Geraldine³^ORCID,Teixeira Miguel C.¹²^ORCID

Affiliation:

1. Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Lisbon, Portugal

2. iBB-Institute for Bioengineering and Biosciences, Biological Sciences Research Group, Instituto Superior Técnico, Lisbon, Portugal

3. School of Biomedical and Biomolecular Sciences, Conway Institute, University College Dublin, Dublin, Ireland

4. Medical Mycology Research Center, Chiba University, Chiba, Japan

Abstract

The ability to acquire azole resistance is an emblematic trait of the fungal pathogen Candida glabrata . Understanding the molecular basis of azole resistance in this pathogen is crucial for designing more suitable therapeutic strategies. This study shows that the C. glabrata transcription factor (TF) CgRpn4 is a determinant of azole drug resistance. RNA sequencing during fluconazole exposure revealed that CgRpn4 regulates the expression of 212 genes, activating 80 genes and repressing, likely in an indirect fashion, 132 genes.

Funder

Programa Operacional Regional de Lisboa 2020

MEC | Fundação para a Ciência e a Tecnologia

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.00554-20

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