Polyphasic Characterization of a Thermotolerant Siderophilic Filamentous Cyanobacterium That Produces Intracellular Iron Deposits

Author:

Brown Igor I.1,Bryant Donald A.2,Casamatta Dale3,Thomas-Keprta Kathie L.1,Sarkisova Svetlana A.1,Shen Gaozhong2,Graham Joel E.2,Boyd Eric S.4,Peters John W.4,Garrison Daniel H.1,McKay David S.5

Affiliation:

1. Jacobs Engineering/NASA Johnson Space Center, JE 23, P.O. Box 58447, Houston, Texas 77258-8447

2. Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802

3. Department of Biology, University of North Florida, Jacksonville, Florida 32224

4. Department of Chemistry and Biochemistry and The Astrobiology Biogeocatalysis Research Center, Montana State University, Bozeman, Montana 59717

5. NASA Johnson Space Center, KR, 2101 NASA Parkway, Houston, Texas 77058

Abstract

ABSTRACT Despite the high potential for oxidative stress stimulated by reduced iron, contemporary iron-depositing hot springs with circum-neutral pH are intensively populated with cyanobacteria. Therefore, studies of the physiology, diversity, and phylogeny of cyanobacteria inhabiting iron-depositing hot springs may provide insights into the contribution of cyanobacteria to iron redox cycling in these environments and new mechanisms of oxidative stress mitigation. In this study the morphology, ultrastructure, physiology, and phylogeny of a novel cyanobacterial taxon, JSC-1, isolated from an iron-depositing hot spring, were determined. The JSC-1 strain has been deposited in ATCC under the name Marsacia ferruginose , accession number BAA-2121. Strain JSC-1 represents a new operational taxonomical unit (OTU) within Leptolyngbya sensu lato. Strain JSC-1 exhibited an unusually high ratio between photosystem (PS) I and PS II, was capable of complementary chromatic adaptation, and is apparently capable of nitrogen fixation. Furthermore, it synthesized a unique set of carotenoids, but only chlorophyll a . Strain JSC-1 not only required high levels of Fe for growth (≥40 μM), but it also accumulated large amounts of extracellular iron in the form of ferrihydrite and intracellular iron in the form of ferric phosphates. Collectively, these observations provide insights into the physiological strategies that might have allowed cyanobacteria to develop and proliferate in Fe-rich, circum-neutral environments.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference53 articles.

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3. Regulatory Roles for IscA and SufA in Iron Homeostasis and Redox Stress Responses in the Cyanobacterium Synechococcus sp. Strain PCC 7002

4. Bozzola, J. J., and L. D. Russell. 1999. Electron microscopy: principles and techniques for biologists, 2nd ed., p. 670. Jones and Bartlett Publishers, Boston, MA.

5. Braun, V., and M. Braun. 2004. Energy-coupled outer membrane iron transporters, p. 213-236. In R. Benz (ed.), Bacterial and eukaryotic porins. Wiley-VCH, Weinheim, Germany.

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