Direct Real-Time PCR for the Detection and Serotyping of Haemophilus influenzae without DNA Extraction-Reference-Cited by-同舟云学术

Direct Real-Time PCR for the Detection and Serotyping of Haemophilus influenzae without DNA Extraction

Published:2022-04-20 Issue:4 Volume:60 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Marasini Daya¹^ORCID,Whaley Melissa J.¹,Jenkins Laurel T.¹,Hu Fang²,Jiang Wenxin³,Topaz Nadav⁴,Chen Alex¹,Schmink Susanna¹,Dolan Thomas Jennifer¹,Harcourt Brian H.¹,Marjuki Henju¹^ORCID,Wang Xin¹^ORCID

Affiliation:

1. Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA

2. IHRC Inc., Contractor to Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA

3. Weems Design Studio, Inc., Contractor to Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA

4. Bacterial Meningitis Laboratory, Meningitis and Vaccine Preventable Diseases Branch, Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA

Abstract

To monitor the burden and changes in Haemophilus influenzae (Hi) disease, direct real-time PCR (drt-PCR) assays have been developed for Hi detection in monoplex form and its six serotypes in triplex form, directly from cerebrospinal fluid (CSF) specimens. These assays target the phoB gene for the species detection (Hi- phoB ) and serotype-specific genes in region II of the capsule biosynthesis locus (Hi-abf and Hi-cde), identified through comparative analysis of Hi and non-Hi whole-genome sequences.

Funder

HHS | Centers for Disease Control and Prevention

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.02111-21

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