Integration of Rous Sarcoma Virus DNA: a CA Dinucleotide Is Not Required for Integration of the U3 End of Viral DNA-Reference-Cited by-同舟云学术

Integration of Rous Sarcoma Virus DNA: a CA Dinucleotide Is Not Required for Integration of the U3 End of Viral DNA

Published:2008-11-15 Issue:22 Volume:82 Page:11480-11483
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Oh Jangsuk¹²,Chang Kevin W.²³,Hughes Stephen H.²

Affiliation:

1. Laboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, Bethesda, Maryland

2. HIV Drug Resistance Program, National Cancer Institute at Frederick, Frederick, Maryland 21702-1201

3. Office of Technology Transfer, NIH, Rockville, Maryland

Abstract

ABSTRACT The two ends of RSV linear DNA are independently inserted into host DNA by integrase in vivo. We previously showed that the range of U3 sequences that are acceptable substrates for integrase appeared to be greater than the range of acceptable U5 sequences in vivo. We have done additional experiments to determine which U3 sequences are good integrase substrates. On the U3 end, there does not appear to be a stringent requirement for the canonical CA, integrase can efficiently remove three nucleotides, and six nucleotides are sufficient to allow integration with reasonable, albeit reduced, efficiency.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.01353-08

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