Affiliation:
1. Department of Oral Biology, University of Manitoba, Winnipeg, Manitoba, Canada R3E 0W2,1and
2. Department of Medical Microbiology and Immunology, College of Medicine, University of South Florida, Tampa, Florida 336122
Abstract
ABSTRACT
Transposon mutagenesis and marker rescue were used to isolate and identify an 8.5-kb contiguous region containing six open reading frames constituting the operon for the sorbitol
P
-enolpyruvate phosphotransferase transport system (PTS) of
Streptococcus mutans
LT11. The first gene,
srlD
, codes for sorbitol-6-phosphate dehydrogenase, followed downstream by
srlR
, coding for a transcriptional regulator;
srlM
, coding for a putative activator; and the
srlA
,
srlE
, and
srlB
genes, coding for the EIIC, EIIBC, and EIIA components of the sorbitol PTS, respectively. Among all sorbitol PTS operons characterized to date, the
srlD
gene is found after the genes coding for the EII components; thus, the location of the gene in
S. mutans
is unique. The SrlR protein is similar to several transcriptional regulators found in
Bacillus
spp. that contain PTS regulator domains (J. Stülke, M. Arnaud, G. Rapoport, and I. Martin-Verstraete, Mol. Microbiol. 28:865–874, 1998), and its gene overlaps the
srlM
gene by 1 bp. The arrangement of these two regulatory genes is unique, having not been reported for other bacteria.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Cited by
29 articles.
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