Combining Catalyzed Reporter Deposition-Fluorescence In Situ Hybridization and Microautoradiography To Detect Substrate Utilization by Bacteria and Archaea in the Deep Ocean-Reference-Cited by-同舟云学术

Combining Catalyzed Reporter Deposition-Fluorescence In Situ Hybridization and Microautoradiography To Detect Substrate Utilization by Bacteria and Archaea in the Deep Ocean

Published:2004-07 Issue:7 Volume:70 Page:4411-4414
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Teira Eva¹,Reinthaler Thomas¹,Pernthaler Annelie²,Pernthaler Jakob²,Herndl Gerhard J.¹

Affiliation:

1. Department of Biological Oceanography, Royal Netherlands Institute for Sea Research, 1790 AB Den Burg, The Netherlands

2. Max Planck Institute for Marine Microbiology, D-28359 Bremen, Germany

Abstract

ABSTRACT The recently developed CARD-FISH protocol was refined for the detection of marine Archaea by replacing the lysozyme permeabilization treatment with proteinase K. This modification resulted in about twofold-higher detection rates for Archaea in deep waters. Using this method in combination with microautoradiography, we found that Archaea are more abundant than Bacteria (42% versus 32% of 4′,6′-diamidino-2-phenylindole counts) in the deep waters of the North Atlantic and that a larger fraction of Archaea than of Bacteria takes up l -aspartic acid (19% versus 10%).

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.70.7.4411-4414.2004

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